Calculating the melting temperature of PCR primers
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![PDF] Comparison of different melting temperature calculation methods for short DNA sequences | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/a622cb64da206f500ec0bc1195c7fa2387474290/4-Table1-1.png "PDF] Comparison of different melting temperature calculation methods for short DNA sequences | Semantic Scholar")
PDF] Comparison of different melting temperature calculation methods for short DNA sequences | Semantic Scholar
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Calculating the melting temperature of PCR primers
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
